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Keywords Recombined adenovirus;Bone Morphogenetic protein 7;Bone Marrow Stromal Cells;Tissue Engineering; 重组腺病毒;骨形成蛋白7;骨髓基质干细胞;组织工程;
Keywords recombinant adenovirus;hepatitis E virus;vaccine; 重组腺病毒;戊型肝炎病毒;疫苗;
Keywords Recombinant adenovirus;Rabies virus;Immune response; 重组腺病毒;狂犬病毒;免疫应答;
Keywords Recombinant adenovirus;Hepatitis C virus;Vaccine; 重组腺病毒;丙型肝炎病毒;疫苗;
Keywords recombinant adenovirus vector;gene therapy;safety; 腺病毒载体;基因治疗;安全性;
Keywords recombinant adenovirus;gene therapy;cosmid;cytosine deaminase;thymidine kinase; 重组腺病毒;基因疗法;粘粒;胞嘧啶脱氨酶;胸苷激酶;
Keywords recombinant plasmid;recombinant adenovirus;hepatocyte growth factor gene;quality control;safety;stability; 重组质粒;重组腺病毒;肝细胞生长因子;质量控制;安全性;稳定性;
Keywords recombinant adenovirus;hepatoma;alpha fetoprotein promoter;gene therapy; 重组腺病毒;肝癌;甲胎蛋白启动子;基因治疗;
The recombinant adenovirus is successfully constructed and can efficient express EWS/FLI1 in PBMC. 重组腺病毒AdEWS/FLI1构建成功 ,并能在PBMC中稳定有效地表达 ,为进一步进行尤文肉瘤的免疫治疗研究奠定了基础。
Objective To construct recombinant adenovirus by homologous recombination in bacteria. 目的通过细菌内同源重组方法构建重组腺病毒。
Conclusion: The recombinant adenovirus generated is E1 and E3 deleted which can be further used in gene therapy in vivo. 结论：所获重组腺病毒为E1，E3缺陷型，可进一步用于基因治疗研究。
RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用减蛋综合征阳性血清在体外中和重组腺病毒，接种HEK293细胞；
The HI titer of the recombinant adenovirus rAd-H1-GFP and rAd-H1-N1 immunized group was 1:243 and 1:279 respectively in 6 weeks after innoculation. 重组腺病毒rAd-H1-GFP和rAd-H1-N1对仔猪的免疫试验结果表明，免疫后2周能够检测到H1N1亚型SIV的HI抗体，免疫后6周，重组腺病毒rAd-H1-GFP免疫组的HI滴度达1：243，而重组腺病毒rAd-H1-N1免疫组的HI滴度达1：279。
Conclusion The recombinant adenovirus containing human BMP-7 gene was successfully constructed by the method of homogenous recombination in cells. 结论应用细胞内同源重组方法成功构建了含人BMP-7基因的重组腺病毒载体。
Objective:To construct a recombinant adenovirus harboring the human insulin gene regulated by the glucose-dependent insulinotropic polypeptide(GIP) promoter. 目的:构建携带葡萄糖依赖性促胰岛素多肽(GIP)启动子控制下的人胰岛素基因的腺病毒载体。
Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. 在不同浓度NC-8-12和奥曲肽作用下;未转染SSTR2基因胰腺癌细胞株的Survivin表达量无明显变化(P>0.;05);转染了SSTR2基因胰腺癌细胞株的Survivin表达量明显下调(P<0
The infl uence of recombinant adenovirus on the growth of breast cancer cell line MCF7 was stu died. Results show that the recombinant adenovirus can inhibit the growth of MCF7. 获得的重组病毒粒子作用于人乳腺癌细胞系MCF7,初步表明重组腺病毒能抑制MCF7的生长。
Keywords recombined milk;yoghurt;substitution ratio; 复原乳;酸奶;替代比例;
Results: The result of restriction showed the direction of the insertion was right and the recombinant adenovirus generated contained the gene of D116H-ANG. 结果：酶切结果显示，重组粘粒中，D116H-ANG插入方向正确,所获重组腺病毒带有ANG衍生物基因。
Keywords recombined cheese;water;dissolved salt;xanthan gum; 重制干酪;水分;溶解盐;黄原胶;